Journal: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy
Article Title: Osteoarthritis patients with high haemoglobin A1c have increased Toll-like receptor 4 and matrix metalloprotease-13 expression in the synovium
doi: 10.2147/DMSO.S209677
Figure Lengend Snippet: Effect of the TLR4 agonist lipopolysaccharide on MMP13 expression and MMP13 production. ( A ). Flow cytometric analysis of cultured synovial tissue-derived cells. X-axis indicates CD45, y-axis indicates CD90. ( B-C ). Effect of the TLR4 agonist lipopolysaccharide on MMP13 expression and MMP13 production. Relative MMP13 expression ( B ) and MMP13 protein concentration in the culture supernatant ( C ) of synovial cells treated with or without lipopolysaccharide were examined by quantitative PCR and enzyme-linked immunosorbent assay, respectively. Data indicate mean ± standard error (n=8). * P <0.05.
Article Snippet: MMP13 protein concentration in the cell supernatant was analyzed using a commercial ELISA kit (Human Pro-MMP-13 Quantikine ELISA Kit, R&D Systems, Inc., Minneapolis, MN, USA).
Techniques: Expressing, Cell Culture, Derivative Assay, Protein Concentration, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay